KEYWORDS
Next Generation Sequencing
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Core Facility
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Molecular Biology
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RNA modifications
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Epitranscriptome
BIO
EDUCATION
- 2000-2005 : phD thesis on Molecular Biology. pHD defended on 14/10/2005, Doctoral School BioSE, Université Henri Poincaré (UHP)– Nancy
- 1999-2000 : Master Thesis in Microbiology, Enzymology and Nutrition, Doctoral School BioSE, UHP – Nancy
EXPERIENCES
- 2018-Present : Head of NGS Core Facility, UMS2008 Ingénierie Biologie Santé en Lorraine (IBSLor), Nancy, France
- 2016-2017 : Technical Head of NGS Core Facility, FR3209 BMCT, Nancy, France
- 2015-2016 : Postdoc, IMoPA lab, UMR7365, Nancy, France. “High-throughput sequencing for identification of RNA modifications”
- 2012-2015 : Postdoc, Centre Hospitalier Régional Universitaire (CHRU) – Nancy, France. “Molecular mechanisms linked to the development of heart failure : identification of novel RNA biomarkers”
- 2007-2012 : Postdoc, mRNA localization and cell polarity lab, Developmental Biology Unit, European Molecular Biology Laboratory (EMBL) – Heidelberg, Germany. “Molecular mechanisms implicated in Drosophila oskar mRNA localization and its translational control”
KNOW-HOW
Know-How here
- Molecular Biology
- Next Generation Sequencing
- RNA specialist
- Expert in the development of high throughput methods to identify RNA modifications (RiboMethSeq, AlkAniline-seq)
KEY PUBLICATIONS
Key publications here
- High Throughput Methods to analyze RNA modifications (Epitranscriptomics)
- Ringeard M, Marchand V, Decroly E, Motorin Y, Bennasser Y. FTSJ3 is an RNA 2′-O-methyltransferase recruited by HIV to avoid innate immune sensing. Nature. 2019 Jan 9. doi: 10.1038/s41586-018-0841-4.
- Marchand V, Ayadi L, Ernst FGM, Hertler J, Bourguignon-Igel V, Galvanin A, Kotter A, Helm M, Lafontaine DLJ, Motorin Y. AlkAniline-Seq: Profiling of m(7)G and m(3)C RNA Modifications at Single Nucleotide Resolution. Angew Chem Int Ed Engl. 2018 Dec 17;57(51):16785-16790. doi: 10.1002/anie.201810946.
- Marchand V, Blanloeil-Oillo F, Helm M, Motorin Y. Illumina-based RiboMethSeq approach for mapping of 2′-O-Me residues in RNA. Nucleic Acids Res. 2016 Sep19;44(16):e135. doi: 10.1093/nar/gkw547.
