molecular medicine

cardiovascular disease


cell therapy



 1982-87            Gradual study Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic, finished with Rector’s price   

1987                 Pharm.D   thesis:“Inhibitory  effect of Ca antagonists on anaphylactic release  of histamine”. Faculty of Pharmacy, Comenius University, Bratislava

1988-92            Postgradual study – Faculty of Medicine, Comenius University. Bratislava, Slovak Republic

1993                 PhD thesis: “Effect of concomitant application of Ca antagonist and magnesium on contractility smooth muscle” Faculty of Medicine, Comenius University. Bratislava,

2003                 Habilitation in Pharmacology:   Thesis: “Calcium Channel Blockers-molecular Pharmacology and new therapeutic mechanism of action.” Faculty of Pharmacy, Comenius University, Bratislava

2009                 Professor of Pharmacology, inauguration by President of Slovak Republic

 Present academic and scientific positions:

 2001     Director of the Laboratory of Clinical and Experimental Pharmacology, International Laser  Centre, Bratislava

2002     Chair of the Division of Pharmacological Propaedeutic. Faculty of Pharmacy, Comenius University Bratislava

Senior Lecturer for Physiological Pharmacology, Faculty of Nature Sciences, Comenius, University, Bratislava

2003     Vice-chair of the Ethical Committee of the Bratislava region

2005     Assessor of Slovak State Institute for Pharmacy Control

2010     Member of Research Board, Charles University, Praha, Czech Republic

2010     Vice-chair of the Ethical Committee Bratislava Region.

2011     Member of Research Board, University of Veterinary Medicine and Pharmacy, Kosice Slovakia

2013     Professor of Pharmacy, University of Veterinary Medicine and Pharmacy, Kosice Slovak Republic

2015     Chair of the Ethical Committee of Association of Innovative Pharmaceutical Industry (AIFP)

2016     Medicines Policy Adviser of Minister of Health

2017     Adviser of the State secretary of Ministry of Education, Science and Sport, 

 Previous positions:

 1992                 MRC of Canada award.  4 months study stay at Department of Pharmacology,     Faculty of Medicine, University of Ottawa, Canada

1993-1994        Postdoctoral stay at laboratory of Dr. B.S.Tuana, foundated by MDC of Canada Department of Pharmacology, Faculty of Medicine, University of Ottawa, Canada

1996-2001        Visiting scientist, Dept. Pharmacology and toxicology, Catholic University of  Louvain, UCL, Brusselles, Belgium

2004               Visiting scientist, (1 month) Research Centre, Sainte Justine Hospital, Montreal, Canada

2005-2011         Head of Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Bratislava.

2009-2014        Dean of Faculty of Pharmacy, Comenius University of Bratislava

    Chair of Research Board of Faculty of Pharmacy, Comenius University Bratislava  Member of Research Board, Comenius University Bratislava

Member of Research Board, Faculty of Pharmacy, Charles University, Hradec Králove, Czech Republic

Member of Research Board, Faculty of Pharmacy, Veterinary and Pharmacy University, Brno Czech Republic

Member of Research Board, Ministry of Health, Slovak Republic

2010-2014         Member of Research Board, Faculty of Chemical and Food Technology, Slovakia

2010-2015         Member of Research Board, Slovak Research and Development Agency 

2011-2015         Member of Research Board, Faculty of Medicine, Comenius University of Bratislava, Slovak Republic

2012-2018         Member/Alternant, Commission for Advanced Medicines, EMA London,

2013-2015        Professor of Pharmacology, Faculty of Medicine, Comenius University of Bratislava

 Supervisor of pregradual and postgradual students

 Defeated thesis for 8   Bachelor of Medicinal devices. 124 Master of Pharmacy, 43 Pharm.D., 2   RN.D.  8   Ph.D. Students   

  in process:   6 Master of Pharmacy, 3 Pharm.D.; 5 Ph.D.

Scientific Publications

Up to March 2019:

>650 Publications  (including: 3 Book chapter, >100 Peer-reviewed papers and  >300 Abstracts from international conferences), 3 Books Co-author, 1 Book Co-editor, and 3 Textbooks

Professional Societies

1989 Slovak Pharmacological Society

2014 British Pharmacological Society

2016 American Heart Association (AHA)

2016 American Association for the Advancement of Science (AAAS)

Invited Lecture:

More than 20 international and 3x Slovak´s departments.

 Scientific grants

 3x international projects 1x FP EU, 10x Slovak grants

 Other relevant information

 1985                Award of Slovak Academic of Science: “The best student science work”

1989-1992         Member of Academic Senate; Medical Faculty Comenius University.       

1995-1996         Secretary of Academic Senate; Faculty of Pharmacy. Comenius University.

2003-2009         Member of Academic Senate of Comenius University.

2015-2017         Member of the Men’s Health EU Advisory Board

 Professional association/affiliation

 Member of Editorial Board: Clinical Osteology, Folia Medica, Czech and Slovak Pharmacy, Farmaceutický     obzor, Lekárnik, Bedeker Zdravia, Recept, Monitor Medicíny



Over the last few years, the genetic and molecular basis of the disease has become an area of enormous interest. In our project, we are aiming to find new diagnostic markers with a high clinical clinical value. In addition to standard statistical methods, molecular methods – RT-PCR, Western blot analysis, Pathfast, ELISA – will be used to meet the targets.

The basic methodical steps of the methodologies used are known to the solving team. Thanks to the variety of methodologies and versatility of the team, we anticipate the successful integration of experimental results into clinical practice to identify the prediction of patient life. Molecular/cell imaging techniques, molecular biology, biochemistry, physiology, pharmacology and diagnostics will be used to target the prediction of cardiovascular injury progression in ISCH patients and ejection fraction randomization factors. The full participation of physicians-clinics allows for the anticipation of a successful potential therapeutic intervention. Obligatory for our molecular medical research will be the optimization of molecular biology methods from experimental conditions into real clinical practice and their subsequent interpretation for the possibility of timely prediction of the disease.

Thorough testing of specific genomic and proteomic methods will be performed in experimental clinical laboratories. The blood samples will be investigated for specific genes, proteins and microRNA, not only in the whole blood, but also in its individual parts (plasma, blood cells).

The decision-makers build on the work accomplishments of the individual members of the team, as well as on their long-term cooperation. Due to the fact that all members of the team are acquainted with each other, they are able to collaborate effectively and apply methodologies identifying molecular mechanisms and clinical aspects of individual patients involved in pathophysiological cascades of cardiovascular disease with targeting to ISCH with subsequent correlation of pharmacotherapy. For this purpose, RT-PCR, Western Blot Analysis, Immunology (ELISA) and Pathfast will be used. This project involves cooperation not only with the faculty of the Comenius University and the NICD clinic, but also with the unique international connection of experimental and clinical pharmacologists and doctors from the field of clinical pharmacology and cardiac surgery.

RT – PCR is a technique commonly used in molecular biology to detect RNA expression, measure the amplification of DNA using fluorescent dyes. We analyse with RT-CR microRNAs – miR-1, miR-29b, miR-133a, miR-214, miR-208a, miR-208b, miR-499 and E-selectin, ICAM1, NOS3, PECAM1, VCAM1.

Western blot (WB) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. We analyse with WB proteins E-selectin, ICAM1, NOS3, PECAM1, VCAM1.

ELISA (enzyme-linked immunosorbent assay) is a test that uses antibodies and color change to identify a substance. Performing an ELISA involves at least one antibody with specificity for a particular antigen. We detect with ELISA angiotensin II, renin, ACE, CRP and IL-6 in blood samples.

Pathfast system combines the accuracy of a full-scale lab analyzer with the flexibility of a mobile solution. We detect with Pathfast hsCRP, NT-proBNP, D-dimer and troponin I.

In project we will used bioinformatics and statistic procedures that allow prediction of cardio – relevant microRNAs and specific biomarkers that may be involved in clinical outcomes of IHD. It should create the prediction algorithms, databases and data for data mining procedures.

The last period of our work is focused to stem cells therpapy of Cardiovasuclar diseases – clinical trials.


Huang ZP, Kataoka M, Chen J, Wu G, Ding J, Nie M, Lin Z, Liu J, Hu X, Ma L, Zhou B, Wakimoto H, Zeng C, Kyselovic J, Deng ZL, Seidman CE, Seidman JG, Pu WT, Wang DZ: Cardiomyocyte-enriched protein CIP protects against pathophysiological stresses and regulates cardiac homeostasis.  J Clin Invest. 2015 Nov 2;125(11):4122-34. doi: 10.1172/JCI82423. Epub 2015 Oct 5.

Huang ZP, Ding Y, Chen J1, Wu G, Kataoka M, Hu Y, Yang JH, Liu J, Drakos SG, Selzman CH, Kyselovic J, Qu LH, Dos Remedios CG1, Pu WT, Wang DZ: Long non-coding RNAs link extracellular matrix gene expression to ischemic cardiomyopathy. Cardiovasc Res. 2016 Nov 1;112(2):543-554. doi: 10.1093/cvr/cvw201.

Jinghai Chen, Masaharu Kataoka, Zhan-Peng Huan, Fei Gu, Jian Ding, Feng Gao, Tian Liang, Ning Liu, Feng Zhang, Qing Ma, Yingchao Wang, Mingming Zhang, Xiaoyun Hu , Jan Kyselovic, William T. Pu, Jian’an Wang, Da-Zhi Wang:Therapeutic role of miR-19a/19b in cardiac regeneration and protection from myocardial infarction” reference number: NCOMMS-17-22108C, Nature Communications, akceptované 2019.

Szobi, Adrián [UKOFAFT] (35%) – Goncalvesová, Eva (5%) – Varga, Zoltán (5%) – Leszek, Przemyslaw (5%) – Kusmierczyk, Mariusz (5%) – Hulman, Michal (5%) – Kyselovič, Ján [UKOFAFT] (5%) – Ferdinandy, Peter (5%) – Adameová, Adriana [KAUT] [UKOFAFT](30%): Analysis of necroptotic proteins in failing human hearts. In: Journal of Translational Medicine. – Vol. 15, Article Number 86 (2017), Nestr. [7 s.]. – ISSN 479-5876

Kyselovič, Ján (50%) – Varga, Ivan [UKOLFUHE] (50%): Research focused on microRNAs: A link between myocardial remodeling and growth during pathological processes and physical exercises In: Annals of Translational Medicine. – Vol. 5, Suppl. 1 (2017), Art. No. 20 [3 s.]. – ISSN 2305-5839

Rajtík, Tomáš [UKOFAFT] (15%) – Goncalvesová, Eva (10%) – Varga, Zoltán V. (10%) – Przemyslaw, Leszek (10%) – Kusmierczyk, Mariusz (10%) – Hulman, Michal (10%) – Kyselovič, Ján [UKOFAFT] (10%) – Adameová, Adriana [KAUT] [UKOFAFT] (15%) – Ferdinandy, Peter (10%): Posttranslational modifications of calcium/calmodulin-dependent protein kinase II? and its downstream signaling in human failing hearts.  In: American Journal of Translational Research. – Vol. 9, No. 8 (2017), s. 3573-3585. – ISSN 1943-8141

Polakovičová, Mája [UKOTVDKCH] (40%) – Musil, Peter (10%) – Laczo, Eugen [UKOTVKLA] (10%) – Hamar, Dušan [UKOTVKKA] (20%) – Kyselovič, Ján [UKOFAFT] (20%): Circulating microRNAs as potential biomarkers of exercise response. Lit. 130 zázn. In: International Journal of Molecular Sciences. – Roč. 17, č. 10, č. čl. 1553, (2016), s. 1-20. ISSN 1422-0067

Schuessler-Lenz, Martina (5%) – Beuneu, Claire (5%) – Menezes-Ferreira, Margarida (5%) – Jekerle, Veronika (5%) – Bartunek, Jozef (5%) – Chamuleau, Steven (5%) – Celis, Patrick (5%) – Doevendans, Pieter (5%) – O’Donovan, Maura (5%) – Hill, Jonathan(5%) – Hystad, Marit (5%) – Jovinge, Stefan (5%) – Kyselovič, Ján [UKOFAFT] (5%) – Lipnik-Stangelj, Metoda (5%) – Maciulaitis, Romaldas (5%) – Prasad, Krishna (4%) – Samuel, Anthony (4%) – Tenhunen, Olli (4%) – Tonn, Torsten (4%) – Rosano, Giuseppe (3%) -Zeiher, Andreas (3%) – Salmikangas, Paula (3%): Cell-based therapies for cardiac repair: a meeting report on scientific observations and European regulatory view pointsIn: European Journal of Heart Failure. – Vol. 18, No. 2 (2016), s. 133-141. – ISSN 1388-9842

Salmikangas, Paula (6%) – Menezes-Ferreira, Margarida (6%) – Reischl, Ilona (6%) – Tsiftsoglou, Asterios (6%) – Kyselovič, Ján [UKOFAFT] (10%) – Borg, John Joseph (6%) – Ruiz, Sol (6%) – Flory, Egbert (6%) – Trouvin, Jean-Hugues (6%) – Celis,Patrick (6%) – Ancans, Janis k (6%) – Timon, Marcos (6%) – Pante, Guido (6%) – Sladowski, Dariusz (6%) – Lipnik-Stangelj, Metoda (6%) – Schneider, Christian K. (6%): Manufacturing, characterization and control of cell-based medicinal products: challengingparadigms toward commercial use Lit. 63 zázn. In: Regenerative Medicine. – Vol. 10, No. 1 (2015), s. 65-78. – ISSN 1746-0751

Kyselovič, Ján [UKOFAFT] – Martinka, Peter – Baťová, Zuzana – Gažová, Andrea [UKOLFFAU] – Godfraind, Theophile: Calcium channel blocker inhibits western-type diet-evoked atherosclerosis development in apoEdeficient mice(S) In: Journal of Pharmacology and Experimental Therapeutics. – Vol. 315, No. 1 (2005), s. 320-328. – ISSN 0022-3565

Kyselovič, Ján [UKOFAFT] – Křenek, Peter, ml. [UKOFAFT] – Wibo, Maurice – Godfraind, Theophile: Effects of amlodipine and lacidipine on cardiac remodelling and renin production in salt-loaded stroke-prone hypertensive rats In: British Journal of Pharmacology. – Vol. 134, No. 7 (2001), s. 1516-1522. – ISSN 0007-1188

Kyselovič, Ján [UKOFAFT] – Morel, Nicol – Wibo, Maurice – Godfraind, Theophile: Prevention of Salt-Dependent Cardiac Remodeling and Enhanced Gene Expression in Stroke-Prone Hypertensive Rats by the Long-Acting Calcium Channel Blocker Lacidipine In: Journal of Hypertension. – Vol. 16, No. 10 (1998), s. 1515-1522. – ISSN 0263-6352

Kyselovič, Ján [UKOFAFT] – Leddy, J. J. – Ray, A. – Wigle, J. – Tuana, B. S.: Temporal differences in the induction of dihydropyridine receptor subunits and ryanodine receptors during skeletal muscle development: Journal of Biological Chemistry. – Vol. 269, No. 34 (1994), s. 21770-21777. – ISSN 0021-9258