Embryonic stem cells



Endogenous Retrovirus



Diana holds a BS degree in Biochemistry and a PhD in Cellular Biology by the University of Zaragoza (Spain). Her thesis was focused on the study of epigenetic regulation of endogenous retroviruses in embryonic stem cells. In 2013 she moved to Icahn School of Medicine at Mount Sinai (New York, USA) where she continued exploring pluripotency from a proteomic perspective, and became interested in studying the interplay of epigenetic regulators with RNA. Recently, she has been appointed principal investigator at the Center for Research in Molecular Medicine and Chronic Diseases (CiMUS, University of Santiago de Compostela, Spain) with a Ramón y Cajal award, where she is focussing on the interplay of epigenetics and epitranscriptomics in stem cells and ageing.


Embryonic stem cells

Somatic cell reprogramming





1. Garcia-Outeiral V, De La Parte C, Fidalgo M, Guallar D. “The Complexity of TET2 Functions in Pluripotency and Development”. Front Cell Dev Biol. 2021 Jan 18. doi:10.3389/fcell.2020.630754.

2. Guallar D*,#, Fuentes-Iglesias A#, Souto Y, Ameneiro C, Freire-Agulleiro O, Pardavila JA, Escudero A, Garcia-Outeiral V, Moreira T, Saenz C, Xiong H, Liu D, Xiao S, Hou Y, Wu K, Torrecilla D, Hartner JC, Blanco MG, Lee LJ, López M, Walkley CR, Wang J*, Fidalgo M*. “ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress”. Cell Stem Cell. 2020 Aug 6;27(2):300-314.e11. doi: 10.1016/j.stem.2020.04.016. (#Co-first, *Co-corresponding authors). 

3. Lan J, Rajan N, Bizet M, Penning A, Singh NK, Guallar D, Calonne E, Li Greci A, Bonvin E, Deplus R, Hsu PJ, Nachtergaele S, Ma C, Song R, Fuentes-Iglesias A, Hassabi B, Putmans P, Mies F, Menschaert G, Wong JJL, Wang J, Fidalgo M, Yuan B, Fuks F. “Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation”. Nat Commun 2020 Oct 2;11(1):4956. doi: 10.1038/s41467-020-18729-6.

4. Fuentes-Iglesias A, Garcia-Outeiral V, Pardavila JA, Wang J*, Fidalgo M*, Guallar D*. “An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro”.  STAR Protoc 2020 Sep 18;1(2):100093. doi: 10.1016/j.xpro.2020.100093. (*Co-corresponding authors)

5. Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. (2018). “RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells” Nat Genet, 50(3):443-451.

6. Fidalgo M, Huang X*, Guallar D*, Sanchez-Priego C, Valdes VJ, Saunders A, Ding J, Wu WS, Clavel C, Wang J. (2016) “Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States” Cell Stem Cell. 19 – (3), pp. 355 – 69.

7. Guallar D, Wang J. (2016) “Taking the RISC of exiting naïve pluripotency”. Genome Biol. 17, pp. 104 – 107.

8. Gingold J*, Fidalgo M*, Guallar D, Lau Z, Sun Z, Zhou H, Faiola F, Huang X, Lee DF, Waghray A, Schaniel C, Felsenfeld DP, Lemischka IR, Wang J. (2014) “A genome-wide RNAi screen identifies opposing functions of Snai1 and Snai2 on the Nanog dependency in reprogramming” Mol Cell. 56 – (1), pp. 140 – 152.

9. Guallar D and Wang J. (2014) “RNA-binding proteins in pluripotency, differentiation, and reprogramming”. Front Biol (Beijing). 9 – 5, pp. 389 – 409.

10. Guallar D, Pérez-Palacios R, Maria Climent; Ignacio Martínez; Ana Larraga; Marta Fernández Juan; Cesar Vallejo; Pedro Muniesa; Jon Schoorlemmer. (2012) “Expression of endogenous retroviruses is negatively regulated by the pluripotency marker Rex1/Zfp42”.Nucleic Acids Res. 40 – 18, pp. 8993 – 9007.