KEYWORDS
Post-transcriptional modifications
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RNA decay
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methylation
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multiprotein complexes
Marc Graille studied as a Bachelor student in Molecular Biology, Biochemistry and cell biology at University of Montpellier (France). He received a master degree and a PhD in protein engineering and structural biology from University of Paris-Sud (Orsay, France) under the supervision of Dr Bernard Gilquin at CEA Saclay (France). He did his post-doc in Herman van Tilbeurgh’s team (University of Paris-Sud, Orsay, France), working on yeast structural genomics but also on protein complexes involved in mRNA translation.
He was hired as a CNRS permanent researcher in 2005 to work in Herman van Tilbeurgh’s team and and since then, he develops his own research topics linked to eukaryotic mRNA translation and degradation.
In 2012, he joined the Laboratory of Biochemistry at Ecole Polytechnique (Palaiseau, France) to create his own team on the same topics and with the support of CNRS-INSERM ATIP-Avenir program.
Marc Graille’s team particularly pursues 2 main objectives:
- Deciphering the role of post-transcriptional/translational modifications of different molecules (tRNAs, rRNAs and proteins) involved in mRNA translation and hence on cell development.;
- Understanding the molecular mechanisms responsible for the recruitment of mRNA decay enzymes to their targets.
Marc Graille a Biochemist with strong expertise in biochemistry, biophysics, crystallization, and X-ray protein crystallography.
His team has expertise in molecular biology, protein expression in prokaryotic systems, protein purification, enzymology studies, RNA production in vitro, characterization of complex formation by various techniques including microcalorimetry (ITC), fluorescence, SEC-MALLS and X-ray structural biology.
The team has also developped skills in genetics in the following model systems: S. cerevisiae and the Haloferax volcanii archaea.
Finally, the team is implementing studies in human cells using CrispR-Cas9 techniques, transfections, …
– Charenton C; Gaudon-Plesse C; Fourati Z; Taverniti V; Back R; Kolesnikova O; Séraphin B*; Graille M*. (2017), A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5’-3’ mRNA exonuclease in yeast. PNAS, 114(45), E9493-9501.
– Bourgeois G; Létoquart J; Tran N.V; Graille M*. (2017) Trm112, a protein activator of methyltransferases modifying actors of eukaryotic translational apparatus. Biomolecules, 7(1), 7.
– Bourgeois G; Marcoux J; Saliou JM; Cianférani S; Graille M*. (2017), Activation mode of the eukaryotic m2G10 tRNA methyltransferase Trm11 by its partner protein Trm112. Nucleic Acids Res, 45(4), 1971-1982.
– Charenton C; Taverniti V; Gaudon-Plesse C; Back R; Séraphin B; Graille M*. (2016), Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator. Nat. Struct. Mol. Biol. 23(11), 982-986.
– Létoquart J; Tran N.V; Caroline V; Aleksandrov A; Lazar N; van Tilbeurgh H; Liger D; Graille M*. (2015) Insights into molecular plasticity in protein complexes from Trm9-Trm112 tRNA modifying enzyme crystal structure. Nucleic Acids Res, 43(22), 10989-11002.
– Létoquart J; Huvelle E; Wacheul L; Bourgeois G; Zorbas C; Graille M*; Heurgué-Hamard V*; Lafontaine D.L.J.* (2014) Structural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes. PNAS, 111(51), E5518-26.
– Graille M*, Séraphin B. (2012) Surveillance pathways rescuing eukaryotic ribosomes lost in translation. Nat. Rev. Mol. Cell Biol., 13, 727-735
– van den Elzen AMG, Henri J, Lazar N, Gas-Lopez M, Durand D, Lacroute F, NicaiseM, van Tilbeurgh H, Séraphin B*, Graille M*. (2010) Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways. Nat. Struct. Mol. Biol. 17(12), 1446-53.
*: Corresponding author.
